Automatic chemiluminescence instrument is a medical laboratory instrument to make immune analysis through testing human serum or plasma.

First, analyte in specimen reacts with alkaline phosphatase to form complex, and then the complex combines with antigen or antibody which coated in the microplate.

Second, wash to separate the unreacted free ingredients.

Third, join the substrate CSPD, and take advantage of free energy of chemical reactions to motivate the intermediates. Then energy releases in the form of photons and light signal will be converted to electrical signals when photons go through hotomultiplier tubes.

Last, make quantitative analysis in according to the mathematical model set up by standard substance, to aid in clinical diagnosis.